Wireless sensor network (WSN) has numerous sensor nodes connected to sink nodes creating a network that is both dynamic and resource limited. The ever-changing environment and the sensor nodes' limited resources make it difficult to determine the best and most secure network structure. The data relay paths are one of the key issues with data transfers in WSNs strategy. In these situations, meeting the quality-of-service requirements is essential. To overcome these challenges and to find the shortest path Eurygaster optimized secure shortest path routing (EO-SSPR) approach has been proposed. The goal of EO-SSPR is to determine the shortest path while ensuring the secure data transmission. The sensor data collected from the patient is DNA encrypted and further encryption is done with the help of Crystal Kyber encryption. Shortest path is determined with the help of Eurygaster optimization. With the help of key generated, DNA decryption is performed further and the original signal data is finally recovered. The authorized user finally receives the original signal data via the shortest path determined. The encryption and decryption time of the proposed EO-SSPR is 12.03%, 8.57%, 4.01% better than DynCH, SEAMHR, SPSRN respectively.